|Application ||WB, IHC-P, E|
|Other Accession||NP_783163, 28373194|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 45 kDa |
Observed: 48 kDa
|Application Notes||ADRM1 antibody can be used for detection of ADRM1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||ADRM1; ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||ADRM1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ADRM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.|
|Cellular Location||Cytoplasm. Nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
The adhesion-regulating molecule 1 (ADRM1) is thought to be involved in protein recruitment and cell adhesion and functions as a proteasomal ubiquitin receptor (1). ADRM1 promotes the activity of UCH-L5 and plays a role in lymphocyte homing. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37, perhaps serving as a mechanism to accelerate ubiquitin chain disassembly (2,3). ADRM1 expression is induced by IFN-gamma in some cancer cell lines and its expression is upregulated in other metastatic cells, suggesting a role in carcinogenesis (4,5).
Husnjak K, Elsasser S, Zhang N, et al. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008; 453:481-8.
Yao T, Song L, Xu W, et al. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nat. Cell Biol. 2006; 8:994-1002.
Qiu XB, Ouyang SY, Li CJ, et al. hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37. EMBO J. 2006; 25:5742-53.
Al-Shami A, Jhaver KG, Vogel P, et al. Regulators of the proteasome pathway, Uch37 and Rpn13, play distinct roles in mouse development. PLoS One 2010; 5:e13654.
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