|Application ||WB, IHC, IF, E|
|Other Accession||NP_001523, 38708299|
|Calculated MW||Predicted: 50 kDa; Observed: 50 kDa|
|Application Notes||SLC29A2 antibody can be used for detection of SLC29A2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SLC29A2; SLC29A2 antibody is human specific. SLC29A2 antibody is predicted to not cross-react with other SLC29 proteins.|
|Reconstitution & Storage||SLC29A2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SLC29A2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DER12, ENT2, HNP36|
|Function||Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.|
|Cellular Location||Basolateral cell membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein. Note=Localized at the basolateral cell membrane in polarized MDCK cells|
|Tissue Location||Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues|
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Provided below are standard protocols that you may find useful for product applications.
SLC29A2 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A2 is a transmembrane glycoprotein that mediates the cellular uptake of nucleosides from the surrounding medium (3). As a nucleoside transporter, SLC29A2 plays an important role in the uptake of nucleoside-based anti-cancer drugs; polymorphisms of point mutations in the gene encoding this protein may affect the efficacy of these drugs (4).
Williams JB and Lanahan AA. A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein. Biochim. Biophys. Res. Commun. 1995; 213:325-33.
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Mangravite LM, Xiao G, and Giacomini KM. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am. J. Physiol. Renal Physiol. 284:F902-10.
Owen RP, Lagpacan LL, Taylor TR, et al. Functional characterization and haplotype analysis of polymorphisms in the human equilabrative nucleoside transporter, ENT2. Drug Metab. Dispos. 2006; 34:12-5.
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