|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_060814, 148596922|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 52 kDa |
Observed: 57 kDa
|Application Notes||SLC29A3 antibody can be used for detection of SLC29A3 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SLC29A3; SLC29A3 antibody is human, mouse and rat reactive. At least two isoforms of SLC29A3 are known to exist; this antibody will detect both isoforms. SLC29A3 antibody is predicted to not cross-react with other SLC29 proteins.|
|Reconstitution & Storage||SLC29A3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SLC29A3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). Mediates transport of adenine, adenosine and uridine, as well as several nucleoside analog drugs, such as anticancer and antiviral agents, including cladribine, cordycepin, tubercidin and AZT. Does not transport hypoxanthine.|
|Cellular Location||Membrane; Multi-pass membrane protein. Late endosome membrane. Lysosome membrane. Note=Observed in a punctate intracellular pattern showing partial colocalization with late endosomes/lysosomes. Not detected at the cell surface|
|Tissue Location||Widely expressed in both adult and fetal tissues. Highest levels in placenta, uterus, ovary, spleen, lymph node and bone marrow. Lowest levels in brain and heart|
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Provided below are standard protocols that you may find useful for product applications.
SLC29A3 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A3 is a transmembrane glycoprotein that localizes to the lysosomal membrane and is a broad selectivity, low affinity nucleoside transporter (3). Mutations in the SLC29A3 gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism (4). A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus (5).
Hyde RJ, Cass CE, Young JD, et al. The ENT family of eukaryotic nucleoside and nucleobase transporters: recent advances in the investigation of structure/function relationships and the identification of novel isoforms. Mol. Membr. Biol. 2001; 18:53-63.
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Baldwin SA, Yao SY, Hyde RJ, et al. Functional characterization of novel human and mouse equilibrative nucleoside transporters (hENT3 and mENT3) located in intracellular membranes. J. Biol. Chem. 2005; 280:15880-7.
Priya TP, Philip N, Molho-Pessach V, et al. H syndrome: novel and recurrent mutations in SLC29A3. Br. J. Dermatol. 2010; 162:1132-4.
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