|Application ||WB, E|
|Other Accession||NP_056012, 155029546|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 98 kDa |
Observed: 95 kDa
|Application Notes||PACS2 antibody can be used for detection of PACS2 by Western blot at 1 - 2 µg/ml.|
|Target/Specificity||PACS2; PACS2 antibody is human, mouse and rat reactive. At least three isoforms of PACS2 are known to exist; this antibody will detect all three. PACS2 antibody is predicted to not cross-react with PACS1.|
|Reconstitution & Storage||PACS2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PACS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, including the apposition of mitochondria with the ER and ER homeostasis. In addition, in response to apoptic inducer, translocates BIB to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated BID, the release of cytochrome c, the activation of caspase-3 thereby causing cell death. May also be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.|
|Cellular Location||Endoplasmic reticulum lumen. Mitochondrion|
|Tissue Location||Broadly expressed, with greatest levels in skeletal muscle followed by heart, brain, pancreas and testis|
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Provided below are standard protocols that you may find useful for product applications.
PACS2 (phosphofurin acidic cluster sorting protein-2), PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS family (1,2). It is expressed in a broad range of tissues with highest expression in skeletal muscle, brain, heart, testis and pancreas (2,). PACS2 interacts with Polycystin-2 and BID and functions as a sorting protein that regulates mitochondria-ER communication and is thought to be involved in ion channel trafficking, specifically direct cluster-containing ion channels to distinct subcellular compartments (3-5).
Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
Brasacchio D, Noori T, House C, et al. A functional genomics screen identifies PCAF and ADA3 as regulators of human granzyme B-mediated apoptosis and Bid cleavage. Cell Death Differ. 2014; 21:748-60.
Werneburg NW, Bronk SF, Guicciardi ME, et al. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein-induced lysosomal translocation of proapoptotic effectors is mediated by phosphofurin acidic cluster sorting protein-2 (PACS-2). J. Biol. Chem. 2012; 287:24427-37.
You H and Thomas G. A homeostatic switch in PACS-2 links membrane traffic to TRAIL-induced apoptosis. Cell Cycle 2009; 8:2679-80.
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