|Application ||WB, IHC, IF, E|
|Other Accession||NP_004902, 4758304|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 71 kDa; Observed: 75 kDa|
|Application Notes||ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||PDIA4; ERP72 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||ERp72 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ERp72 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).|
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Provided below are standard protocols that you may find useful for product applications.
Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (1,2). ER stress protein 72 (ERp72), also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement (3). It plays a role in the formation and isomerization of disulfide bonds (3,4).
Huppa JB and Ploegh HL. The eS-Sence of -SH in the ER. Cell 1998; 92:145-8.
Ellgaard L and Ruddock LW. The human protein disulphide isomerase family: substrate interactions and functional properties. EMBO Rep. 2005; 6:28-32.
Mazzarella RA, Srinivasan M, Haugejorden SM, et al. ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. J. Biol. Chem. 1990; 265:1094-101.
Satoh M, Shimada A, Keino H, et al. Functional characterization of 3 thioredoxin homology domains of ERp72. Cell Stress Chaperones 2005; 10:278-84.
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