|Application ||WB, IHC, IF, E|
|Other Accession||NP_002068, 4504063|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 84 kDa; Observed: 120 kDa|
|Application Notes||GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||GOLGA1; GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.|
|Reconstitution & Storage||GOLGIN97 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||GOLGIN97 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in maintaining Golgi structure.|
|Cellular Location||Golgi apparatus, Golgi stack membrane; Peripheral membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren’s syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).
Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren’s syndrome. Arthritis Rheum. 1997; 40:1693-702.
Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104.
Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43.
Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.
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