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LRRC8A Antibody

     
  • WB - LRRC8A Antibody ASC11942
    Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 µg/ml.
  • IHC - LRRC8A Antibody ASC11942
    Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 µg/mL.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q8IWT6
Other Accession NP_062540, 62241040
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 89 kDa; Observed: 95 kDa
Application Notes LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.
Additional Information
Gene ID 56262
Target/Specificity LRRC8A; LRRC8A antibody is human, mouse and rat reactive.
Reconstitution & Storage LRRC8A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsLRRC8A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LRRC8A
Synonyms KIAA1437, LRRC8, SWELL1
Function Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine (PubMed:24725410, PubMed:24790029, PubMed:26530471, PubMed:26824658). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658). Can form functional channels by itself (in vitro) (PubMed:26824658). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746). Also required for T-cell development (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein. Note=The leucine-rich repeat (LRR) domain is on the cytoplasmic side of the cell membrane
Tissue Location Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells Expressed on T-cells as well as on B-lineage cells
Research Areas
Citations (0)

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Background

The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).

References

Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13.
Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52.
Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.

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$ 320.00
Cat# ASC11942
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