|Application ||WB, IHC, E|
|Other Accession||NP_062540, 62241040|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 89 kDa; Observed: 95 kDa|
|Application Notes||LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Target/Specificity||LRRC8A; LRRC8A antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||LRRC8A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||LRRC8A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA1437, LRRC8, SWELL1|
|Function||Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. It is unclear whether LRRC8A constitutes a pore-forming subunit or whether it is closely associated with the pore and mediates channel properties such as ion selectivity. Involved in B-cell development: required for the pro-B cell to pre-B cell transition. Also required for T-cell development.|
|Cellular Location||Cell membrane; Multi- pass membrane protein|
|Tissue Location||Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells Expressed on T-cells as well as on B-lineage cells|
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Provided below are standard protocols that you may find useful for product applications.
The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).
Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13.
Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52.
Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.
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