|Application ||WB, IHC, E|
|Other Accession||NP_001124170, 194733735|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 90, 93, 101 kDa; Observed: 93 kDa|
|Application Notes||TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 µg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Target/Specificity||TRPC3; TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.|
|Reconstitution & Storage||TRPC3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||TRPC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5- triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.|
|Cellular Location||Membrane; Multi-pass membrane protein.|
|Tissue Location||Expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61.
Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41.
Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
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