|Application ||WB, E|
|Other Accession||NP_689596, 134288890|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 97 kDa; Observed: 94 kDa|
|Application Notes||DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||DIS3L2; DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L.|
|Reconstitution & Storage||DIS3L2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DIS3L2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.|
|Cellular Location||Cytoplasm. Cytoplasm, P-body|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The exosome is involved in a multitude of cellular RNA processing and degradation events (1). DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2). DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3). DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4). DIS3L2 mutations have also been associated with sporadic Wilms tumors (3).
Chen CY, Gherzi R, Ong SE, et al. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 2001; 107:451-64.
Brouwer R, Allmang C, Raijmakers R, et al. Three novel components of the human exosome. J. Biol. Chem. 2001; 276:6177-84.
Astuti D, Morris MR, Cooper WN, et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat. Genet. 2012; 44:277-84.
Malecki M, Viegas SC, Carneiro T, et al. The exonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway. EMBO J. 2013; 32:1842-54.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.