|Application ||WB, E|
|Other Accession||NP_001258, 153251229|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 38 kDa |
Observed: 36 kDa
|Application Notes||Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 µg/ml.|
|Target/Specificity||CHAD; Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.|
|Reconstitution & Storage||Chondroadherin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||Chondroadherin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes attachment of chondrocytes, fibroblasts, and osteoblasts. This binding is mediated (at least for chondrocytes and fibroblasts) by the integrin alpha(2)beta(1). May play an important role in the regulation of chondrocyte growth and proliferation (By similarity).|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Present in chondrocytes at all ages.|
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Provided below are standard protocols that you may find useful for product applications.
Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4). Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2,4,5).
Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54.
Haglund L, Tillgren V, Addis L, et al. Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment. J. Biol. Chem. 2011; 286:3925-34.
Mansson B, Wenglen C, Morgelin M, et al. Association of chondroadherin with collagen type II. J. Biol. Chem. 2001; 276:32883-8.
Camper L, Heinegard D, and Lundgren-Akerlund E. Integrin alpha2beta1 is a receptor for the cartilage matrix protein chondroadherin. J. Cell Biol. 1997; 138:1159-67.
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