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ORAI3 Antibody [2H2G9]
![WB - ORAI3 Antibody [2H2G9] ASC11997](/assets/uploads/products/201512/ASC11997_WB_2.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9BRQ5 |
| Other Accession | Q9BRQ5, 93129 |
| Reactivity | Human, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone Names | 2H2G9 |
| Calculated MW | 31499 Da |
| Application Notes | ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Gene ID | 93129 |
|---|---|
| Target/Specificity | A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3. |
| Reconstitution & Storage | ORAI3 monoclonal antibody can be stored at -20℃, stable for one year. |
| Precautions | ORAI3 Antibody [2H2G9] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ORAI3 |
|---|---|
| Synonyms | TMEM142C |
| Function | Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1. |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Expressed in both naive and effector T helper cells with higher levels in effector cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.
References
Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.
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