|Application ||WB, IHC-P, E|
|Other Accession||NP_000607, 920|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||51111 Da|
|Application Notes||CD4 antibody can be used for detection of CD4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL|
|Target/Specificity||Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.|
|Reconstitution & Storage||CD4 monoclonal antibody can be stored at -20℃, stable for one year.|
|Precautions||CD4 Antibody [9H5A8] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum|
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Provided below are standard protocols that you may find useful for product applications.
CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.
Bowers K, Pitcher C, and Marsh M. CD4 : a co-receptor in the immune response and HIV infection. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.
Arthos J, Deen KC, Chaikin MA, et al. Identification of the residues in human CD4 critical for the binding of HIV. Cell 1989; 57:469-81.
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