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DDDDK Antibody [1D1B12] (biotin)

     
  • WB - DDDDK Antibody [1D1B12] (biotin) ASC12045
    Western blot analysis of (1) 1000ng, (2) 500ng, (3) 250ng, and (4) 125ng of DDDDK-tagged recombinant protein (GGP1) with Biotin-DDDDK-tag antibody at 0.5 µg/ml.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P60709
Other Accession 60
Host Mouse
Clonality Monoclonal
Isotype IgG
Calculated MW 41737 Da
Application Notes Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 µ/ml.
Additional Information
Gene ID 60
Other Names Biotin-DDDDK, DDDDK, FLAG, FLAGtag, flag-tag
PrecautionsDDDDK Antibody [1D1B12] (biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas
Citations (0)
citation

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Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.

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$ 379.00
Cat# ASC12045
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