SAP102 Antibody
SAP102 Antibody, Clone S19-2
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IP, ICC/IF, AM |
---|---|
Primary Accession | Q62936 |
Other Accession | NP_113827.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Rat SAP102 Monoclonal IgG1 |
Target/Specificity | Detects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110. |
Other Names | DLG3 Antibody, MRX90 Antibody, NEDLG Antibody, XLMR Antibody, Disks large homolog 3 Antibody, PSD-95/SAP90-related protein 1 Antibody, Synapse-associated protein 102 Antibody, SAP-102 Antibody |
Clone Names | S19-2 |
Immunogen | Fusion protein amino acids 1-120 of rat SAP102 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml was sufficient for detection of Sap102 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary. |
Cellular Localization | Basolateral Cell Membrane | Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density | Growth Cone |
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Provided below are standard protocols that you may find useful for product applications.
Background
Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homology 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
References
1. Muller B.M., et al. (1996) Neuron 17: 255-265.
2. Lau L. F., et al. (1996) J. Biol. Chem. 271: 21622-21628.
3. Cuthbert P.C., et al. (2007) J Neuroscience 27:2673-2682.
4. Lau L.F., et al. (1996) J Biol Chem 274:5782-5790.
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