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AHA1 Antibody

AHA1 Antibody, Clone 25F2.D10

     
  • ICC/IF - AHA1 Antibody ASM10108
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (ASM10108). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10108) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).
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  • IP - AHA1 Antibody ASM10108
    Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (ASM10108). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10108) at 1:1000.
    detail
  • IHC - AHA1 Antibody ASM10108
    Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (ASM10108). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10108) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
    detail
  • WB - AHA1 Antibody ASM10108
    Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (ASM10108). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (ASM10108) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, E
Primary Accession Q8BK64
Other Accession NP_666148.1
Host Rat
Isotype IgG2a Kappa
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa
Target/Specificity Detects ~38kDa. Can run up to 45kDa on SDS Page.
Other Names Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
Clone Names 25F2.D10
Immunogen Mouse Aha1
Purification Protein G Purified
Storage -20ºC
Storage Buffer 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-173 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Endoplasmic Reticulum
Research Areas
Citations (0)
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Background

Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

References

1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

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Cat# ASM10108
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