VAMP1/2 Antibody
VAMP1/2 Antibody, Clone SP-11
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
---|---|
Primary Accession | P23763 |
Other Accession | NP_055046.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Human VAMP1/2 Monoclonal IgG1 |
Target/Specificity | Detects ~20-25kDa. Recognizes VAMP 1 and 2. |
Other Names | SYB1 Antibody, Synaptobrevin Antibody, vesicle associated membrane protein Antibody |
Clone Names | SP-11 |
Immunogen | Crude human synaptic immunoprecipitate |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-179 was sufficient for detection of VAMP in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Mitochondrion | Mitochondrion Outer Membrane | Cytoplasmic Vesicle |
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Provided below are standard protocols that you may find useful for product applications.
Background
VAMP-1, also known as vesicle-associated membrane protein, is an 18kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane (1). Synaptobrevins, Syntakins and the 25kDa synaptosomal associated SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (2). VAMP-1 is also one of the key proteins in the SNARE complex which is involved in regulated exocytosis (3).
References
1. Trimble W.S., Cowan D.M., and Scheller R.H. (1988) Proc Natl Acad Sci USA. 85: 4538-4542.
2. Nabokina S.M. (2001) Bulletin of Experimental Bio and Med. 131(2): 130-131.
3. Berglund L., Hoffman H.J., Dahl R., and Peterson T.E. (1999) Biochem Biophys Research Communicaitons. 264(3): 777-780.
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