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Slo2.2 Antibody

Slo2.2 Antibody, Clone S3-26

     
  • ICC/IF - Slo2.2 Antibody ASM10199
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26 (ASM10199). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (ASM10199) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.
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  • WB - Slo2.2 Antibody ASM10199
    Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26 (ASM10199). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (ASM10199) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
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  • IHC - Slo2.2 Antibody ASM10199
    Immunohistochemistry analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26 (ASM10199). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (ASM10199) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, AM
Primary Accession Q9Z258
Other Accession NP_068625
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat Slo2.2 Monoclonal IgG1
Target/Specificity Detects ~140kDa. Weak human detection.
Other Names KCNT1 Antibody, potassium channel subfamily T member 1 Antibody, bA100C15.2 Antibody, SLACK Antibody, KCa4.1KIAA1422FLJ41282 Antibody
Clone Names S3-26
Immunogen Fusion protein amino acids 1168-1237 of rat Slo2.2 (Slack)
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-323 was sufficient for detection of Slo2.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane
Citations (0)
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Background

Slo2.2 is a novel member of the mammalian Slo potassium channel gene family. Slo2 channels may contribute to the resting potentials of cells that control their basal level of excitability (1). They also have sensors that couple channel activity to the intracellular concentrations of Na+ and Cl- (2).

References

1. Santi C.M., et al. (2006) J Neuroscience, 26(19): 5059-5068.
2. Yuan A., et al. (2003) Neuron 37: 765-773.

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Cat# ASM10199
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