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Kv3.4 Antibody

Kv3.4 Antibody, Clone S72-16

     
  • ICC/IF - Kv3.4 Antibody ASM10211
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (ASM10211). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (ASM10211) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli .
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  • WB - Kv3.4 Antibody ASM10211
    Western Blot analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (ASM10211). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (ASM10211) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
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  • IHC - Kv3.4 Antibody ASM10211
    Immunohistochemistry analysis using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 (ASM10211). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody (ASM10211) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrin-like staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, AM
Primary Accession Q63734
Other Accession NP_001116248.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat Kv3.4 Monoclonal IgG1
Target/Specificity Detects ~70kDa (100kDa in brain due to glycosylation).
Other Names Raw3 Antibody, Voltage-gated potassium channel subunit Kv3.4 Antibody, Potassium voltage-gated channel subfamily C member 4 Antibody
Clone Names S72-16
Immunogen Synthetic peptide amino acids 175-192 of rat Kv3.4
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-335 was sufficient for detection of Kv3.4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane
Citations (0)
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Background

Kv3.4, a member of the Shaw subfamily, may play important roles in maintaining normal function of the corneal epithelium (1). Kv3.4 is also over-expressed in the early stages of Alzheimer’s disease, and therefore represents a novel therapeutic target for this disease (2).

References

1. Wang L., Fyffe R.E.W., and Lu L. (2004) Investigative Ophthalmology and Visual Science. 45: 1796-1803.
2. Angulo E., et al. (2004) J Neurochem. 91(3): 547-557.

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Cat# ASM10211
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