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GABA-A Receptor Delta Antibody

GABA A Receptor Delta Antibody, Clone S151-3

     
  • ICC/IF - GABA-A Receptor Delta Antibody ASM10221
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (ASM10221). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (ASM10221) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.
    detail
  • WB - GABA-A Receptor Delta Antibody ASM10221
    Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (ASM10221). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (ASM10221) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, AM
Primary Accession P18506
Other Accession NP_058985.1
Host Mouse
Isotype IgG2a
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GABA-A Receptor Delta Monoclonal IgG2a
Target/Specificity Detects ~55kDa.
Other Names GABAA-RD Antibody, Gabrd Antibody, MGC105467 Antibody
Clone Names S151-3
Immunogen Synthetic peptide amino acids 15-34 of rat GABA-A-R-Delta
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SMC-345 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane
Research Areas
Citations (0)
citation

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Background

The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).

References

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136.
2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.

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Cat# ASM10221
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