VGLUT1 Antibody
VGLUT1 Antibody, Clone S28-9
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | Q62634 |
Other Accession | NP_446311.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Rat VGLUT1 Monoclonal IgG1 |
Target/Specificity | Detects ~52kDa. No cross-reactivity against VGlut2. |
Other Names | BNPI Antibody, SLC17A1 Antibody, SLC17A1/VGLUT1 Antibody, Solute Carrier family 17 member 7 Antibody, Vesicular glutamate transporter 1 Antibody, VGLUT 1 Antibody, Brain-specific Na(+)-dependent inorganic phosphate cotransporter Antibody |
Clone Names | S28-9 |
Immunogen | Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse |
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Provided below are standard protocols that you may find useful for product applications.
Background
VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
References
1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163.
2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.
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