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VGLUT1 Antibody

VGLUT1 Antibody, Clone S28-9

     
  • WB - VGLUT1 Antibody ASM10228
    Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (ASM10228). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (ASM10228) at 1:1000.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q62634
Other Accession NP_446311.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat VGLUT1 Monoclonal IgG1
Target/Specificity Detects ~52kDa. No cross-reactivity against VGlut2.
Other Names BNPI Antibody, SLC17A1 Antibody, SLC17A1/VGLUT1 Antibody, Solute Carrier family 17 member 7 Antibody, Vesicular glutamate transporter 1 Antibody, VGLUT 1 Antibody, Brain-specific Na(+)-dependent inorganic phosphate cotransporter Antibody
Clone Names S28-9
Immunogen Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse
Research Areas
Citations (0)
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Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

References

1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163.
2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.

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Cat# ASM10228
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