VGlut3 Antibody
VGLUT3 Antibody, Clone S34-34
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC/IF |
---|---|
Primary Accession | Q7TSF2 |
Other Accession | NP_714947.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Rat VGlut3 Monoclonal IgG1 |
Target/Specificity | Detects ~65kDa. |
Other Names | DFNA25 Antibody, Vesicular glutamate transporter 3 Antibody, deafness autosomal dominant 25 Antibody, SLC17A8 Antibody, Solute carrier family 17 member 8 Antibody, VGLUT 3 Antibody, Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8 Antibody |
Clone Names | S34-34 |
Immunogen | Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-397 was sufficient for detection of VGLut3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGlut3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse |
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Provided below are standard protocols that you may find useful for product applications.
Background
Vesicular Glutamate Transporter 3 (VGLUT3) is a multipass membrane protein restricted to synaptic vesicles of glutamergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
References
1. Amilhon B., et al. (2010) J Neurosci. 30(6): 2198-2210.
2. Herzog E., et al. (2004) Neurosci. 123(4): 983-1002.
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