GABA-B Receptor 1 Antibody
GABA B Receptor 1 Antibody, Clone S93A-49
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, ICC |
---|---|
Primary Accession | Q9Z0U4 |
Other Accession | NP_112290.2 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Rat GABA-B Receptor 1 Monoclonal IgG1 |
Target/Specificity | Detects ~115kDa. No cross-reactivity against GABA(B)R2. |
Other Names | GABA-B receptor 1 Antibody, GABA-B-R1 Antibody, GABR1_Human Antibody, Gamma aminobutyric acid receptor 1 Antibody, GB1 Antibody, GPRC3A Antibody |
Clone Names | S93A-49 |
Immunogen | Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-403 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
References
1. Jones K.A,. et al. (2000) Neuropsychopharmacology 23: S41-9.
2. Duthey B., et al. (2002) J Biol Chem. 277: 3236-41.
3. Kaupmann K., et al. (1997) Nature 386: 239-46.
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