AMIGO-1 Antibody
AMIGO1 Antibody, Clone S86-36
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC/IF |
---|---|
Primary Accession | Q86WK6 |
Other Accession | NP_065754.2 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Human AMIGO-1 Monoclonal IgG1 |
Target/Specificity | Detects ~60-80kDa depending on maturity/glycosylation. |
Other Names | AMIGO 1 Antibody, AMIGO1 Antibody, Adhesion molecule with Ig like domain 1 Antibody, Amphoterin-induced protein 1 Antibody, Alivin-2 Antibody, Alivin 2 Antibody, Ali2 Antibody, AMIGO Antibody, KIAA1163 Antibody, Amphoterin induced gene and ORF (Amigo) Antibody, Amphoterin induced protein 1 Antibody, MGC25558 Antibody, OTTHUMP00000013379 Antibody, RP23 89M15.6 Antibody |
Clone Names | S86-36 |
Immunogen | Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cell Membrane | Cell Projection | Axon |
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Provided below are standard protocols that you may find useful for product applications.
Background
The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
References
1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973.
2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270.
3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896.
4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.
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