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4-Hydroxynonenal Antibody

4-Hydroxynonenal Antibody, Clone 12F7

     
  • ICC/IF - 4-Hydroxynonenal Antibody ASM10339
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7 (ASM10339). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (ASM10339) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
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  • WB - 4-Hydroxynonenal Antibody ASM10339
    Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal-BSA using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (ASM10339). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (ASM10339) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, E
Host Mouse
Isotype IgG1
Clonality Monoclonal
Description Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1
Target/Specificity Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Other Names 4-Hydroxynonenal Antibody, 4-Hydroxy Nonenal (4-HNE) Antibody, 4-Hydroxy Nonenal Antibody, 4-HNE Antibody, 4-hydroxy Hexenal Antibody, HNE Antibody, 4-hydroxy-2-nonenal Antibody
Trademark MOLECULAR SIGNATURE®
Clone Names 12F7
Immunogen Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SMC-511 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Cat# ASM10339
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