|Description||Anti-SHEEP IgG (H&L) (DONKEY) Antibody|
|Application ||WB, E, IC|
|Application Note||ELISA 1:20,000-1:100,000|
Western Blot 1:2,000-1:10,000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Sheep IgG whole molecule|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Anti-SHEEP IgG Antibody was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Sheep IgG whole molecule antibody generated in donkey detects specifically Sheep IgG whole molecule. This secondary antibody anti-Sheep is ideal for investigators who routinely perform western blot, immunoprecipitation and more generally immunoassays.
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