|Description||Anti-Human IL-6 (RABBIT) Antibody|
|Application ||WB, IP, E, IC|
|Application Note||ELISA 1:1,000-1:5,000|
Western Blot 1:500-1:2,000
|Physical State||Liquid (sterile filtered)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.|
|Shipping Condition||Dry Ice|
|Purity||Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6.|
|Storage Condition||Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti IL-6 Antibody recognizes IL-6 that is a secreted cytokine with a wide variety of biological functions. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Immunology and Cancer research
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