|Description||Anti-RABBIT IgG F(c) (DONKEY) Antibody|
|Application ||WB, E, IC|
|Application Note||ELISA 1:20,000-1:100,000|
Western Blot 1:2,000-1:10,000
|Target Isotype||IgG F(c)|
|Buffer||0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Rabbit IgG F(c) fragment|
|Reconstitution Volume||2.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Purity||This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab’)2 .|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Rabbit antibody generated in donkey detects specifically rabbit IgG F(s). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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