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Anti-Bovine Serum Albumin BSA Secondary Antibody

Rabbit Polyclonal, Unconjugated

     
  • WB - Anti-Bovine Serum Albumin BSA  Secondary Antibody ASR1522
    Western Blot of Rabbit anti-Bovine Serum Albumin antibody. Lane 1: BSA. Lane 2: None. Load: 50 ng per lane. Primary antibody: BSA antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO-TBS for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for BSA. Other band(s): None.
    detail
  • SPECIFICATION
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  • BACKGROUND
Product Information
Description Anti-BOVINE ALBUMIN (RABBIT) Antibody (BSA)
Host Rabbit
Conjugate Unconjugated
Target Species Bovine
Reactivity Bovine
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype Antiserum
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with Bovine Serum Albumin.
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Bovine Serum Albumin antibody is supplied in the form of monospecific antiserum prepared by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Albumin [Bovine Serum].  Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined.
Storage Condition Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

Anti-Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

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$ 229.00
Cat# ASR1522
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