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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Human IgG (H&L) Pre-Adsorbed Secondary Antibody   

Anti-Human IgG (H&L) Pre-Adsorbed Secondary Antibody

Rabbit Polyclonal, Unconjugated

     
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Product Information
Description Anti-HUMAN IgG (H&L) (RABBIT) Antibody (Min X MOUSE Serum Proteins)
Host Rabbit
Conjugate Unconjugated
Target Species Human
Reactivity Human
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:75,000-1:500,000
Western Blot 1:10,000-1:50,000
Immunochemistry 1:1,000-1:10,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Human IgG was produced by repeated immunization with human IgG whole molecule fragment in rabbit.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed using ELISA minimal cross reaction against Mouse IgG.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
Citations (0)

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Background

Anti-Human antibody generated in rabbit detects specifically human IgG (H&L). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 138.00
Cat# ASR1603
Size:
Quantity:
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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