Anti-Mouse IgG (H&L) (Fluorescein Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Fluorescein (FITC)
|Description||Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Min X Human Serum Proteins)|
|FP Value||6.6 moles Fluorescein (FITC) per mole of IgG|
|Application ||IF, FC|
|Application Note||IF Microscopy 1:500-1:2,500|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Mouse IgG whole molecule|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.|
|Storage Condition||Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Mouse secondary antibody conjugated to FITC is available in a variety of formats. Anti IgG secondary antibody conjugated is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti mouse IgG FITC is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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