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Anti-Human IgA (alpha chain) Secondary Antibody

Rabbit Polyclonal, Unconjugated

  • WB - Anti-Human IgA (alpha chain)  Secondary Antibody ASR1789
    Western Blot of Rabbit Anti-HUMAN IgA (alpha chain) Antibody Lane 1: Human IgA Load: 100 ng per lane Primary antibody: HUMAN IgA (alpha chain) Antibody at 1:1000 for 60 min at RT Secondary antibody: DyLight 649 goat anti-rabbit at 1:20,000 for 30 min at RT Block: 5% BLOTTO 30 min at RT Predicted/Observed size: 60and 28 kDa, 60 and 28 kDa
Product Information
Description Anti-HUMAN IgA (alpha chain) (RABBIT) Antibody
Host Rabbit
Conjugate Unconjugated
Target Species Human
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:5,000-1:20,000
Western Blot 1:500-1:2,000
Immunochemistry 1:250-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgA
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Human IgA alpha heavy chain
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s).  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum.  No reaction was observed against other human heavy or light chain proteins.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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$ 139.00
Cat# ASR1789
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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Ordering Information

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