|Description||Anti-HUMAN IgM (Fc5u) (RABBIT) Antibody|
|Application ||WB, E, IC|
|Application Note||ELISA 1:40,000-1:160,000|
Western Blot 1:500-1:3,000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgM Fc5 µ|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Human IgM Fc5 µ was produced by repeated immunization with Human IgM Fc5 µ fragment in rabbit.|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Human IgM Fc5 µ Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Human IgM Fc5 µ antibody generated in rabbit detects specifically human IgM. This anti-human IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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