|Description||F(ab')2 Anti-DOG IgG [H&L] (RABBIT) Antibody|
|Application ||WB, IHC, E|
|Application Note||ELISA 1:5,000-1:20,000|
Western Blot 1:2,000-1:10,000
|Physical State||Liquid (sterile filtered)|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Dog IgG whole molecule|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||F(ab')2 Anti-Dog IgG (H&L) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Pepsin and anti-Rabbit IgG F(c).|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
F(ab')2 Anti-Dog IgG (H&L) Antibody generated in rabbit detects specifically Dog IgG. This secondary Anti-Dog IgG antibody is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.Anti-Dog IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.
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