F(ab')2 Anti-Cat IgG (H&L) (Fluorescein Conjugated) Secondary Antibody
Rabbit Polyclonal, Fluorescein (FITC)
|Description||F(ab')2 Anti-CAT IgG [H&L] (RABBIT) Antibody Fluorescein Conjugated|
|FP Value||2.7 moles Fluorescein (FITC) per mole of IgG F(ab')2|
|Application ||IF, FC|
|Application Note||FLISA 1:10,000-1:50,000|
IF Microscopy 1:1,000-1:5,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Cat IgG whole molecule|
|Reconstitution Volume||500 µL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Cat IgG and Cat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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