F(ab')2 Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)
|Description||F(ab')2 Anti-RABBIT IgG [H&L] (GOAT) Antibody Alkaline Phosphatase Conjugated|
|Conjugate||Alkaline Phosphatase (Calf Intestine)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:2,000-1:10,000|
Western Blot 1:500-1:2,500
|Physical State||Liquid (sterile filtered)|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Immunogen||Rabbit IgG whole molecule|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Shipping Condition||Wet Ice|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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