|Description||Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins|
|Application ||WB, E, IC|
|Application Note||ELISA 1:40,000-1:200,000|
Western Blot 1:4,000-1:20,000
|Physical State||Liquid (sterile filtered)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Mouse IgG2a heavy chain|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Mouse IgG2a secondary antibody is available in a variety of formats.
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