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Chicken IgG Alkaline Phosphatase

  •  - Chicken IgG Alkaline Phosphatase  ASR2260
    SDS-PAGE of Chicken IgG Whole Molecule Alkaline Phosphatase Conjugated . Lane M: 5 µL Opal Prestained Marker . Lane 1: Reduced Chicken IgG Whole Molecule Alkaline Phosphatase Conjugated . Lane 2: Reduced Chicken IgG F(c) Fragment . Lane 3: Reduced Chicken IgG F(ab) Fragment . Lane 4: Reduced Chicken IgM Whole Molecule . Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.
Product Information
Description CHICKEN IgG whole molecule Alkaline Phosphatase conjugated
Conjugate Alkaline Phosphatase (Calf Intestine)
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunohistochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Species of Origin Chicken
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken Serum and anti-Alkaline Phosphatase (calf intestine).
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Anti-Chicken IgG Antibody detects specifically Chicken IgG whole molecule. Anti-Chicken IgG is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Chicken IgG Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

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$ 175.00
Cat# ASR2260
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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