|Description||MOUSE IgG2a Kappa (κ) isotype control|
|Application Note||FlowCytometry 1:1000-1:5000|
|Physical State||Liquid (sterile filtered)|
|Buffer||0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2|
|Species of Origin||Mouse|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition confirmed by RID.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 3 and µ - IgM, light chain ? and ?.
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