|Description||MOUSE IgM Kappa (κ) isotype control|
|Application Note||FlowCytometry 1:1000-1:5000|
|Physical State||Liquid (sterile filtered)|
|Buffer||0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0|
|Species of Origin||Mouse|
|Preservative||0.1% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Light and heavy chain composition confirmed by Elisa.|
|Storage Condition||Store vial at 4° C prior to opening. Mouse IgM Kappa isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. This isotype control possesses kappa light chains.
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