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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Mouse IgM Kappa (κ) isotype Control   

Mouse IgM Kappa (κ) isotype Control

Monoclonal MMK IgM , Unconjugated

  •  - Mouse IgM Kappa (κ) isotype Control ASR2274
    SDS-PAGE of Mouse IgM Kappa isotype control . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa isotype control . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM ? at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
Product Information
Description MOUSE IgM Kappa (κ) isotype control
Conjugate Unconjugated
Clonality Monoclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note FlowCytometry 1:1000-1:5000
Physical State Liquid (sterile filtered)
Host Isotype IgM
Buffer 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Species of Origin Mouse
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum.  Light and heavy chain composition confirmed by Elisa.
Storage Condition Store vial at 4° C prior to opening.   Mouse IgM Kappa isotype control is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. This isotype control possesses kappa light chains.

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$ 175.00
Cat# ASR2274
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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