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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Human IgG F(c) Secondary Antibody   

Anti-Human IgG F(c) Secondary Antibody

Mouse Monoclonal 3D8.H7.D4 IgG1 kappa , Unconjugated

  • WB - Anti-Human IgG F(c)  Secondary Antibody ASR2310
    Western Blot of Mouse anti-Human Fc antibody. Lane 1: Human IgG. Lane 2: Human Fc. Lane 3: Human F(ab). Lane 4: Human F(ab)2. Lane 5: Human IgM. Lane 6: Human IgM Fc5 µ. Load: 50 ng per lane. Primary antibody: Human Fc monocloanl antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc.
Product Information
Description Anti-HUMAN IgG F(c) (MOUSE) Antibody
Host Mouse
Conjugate Unconjugated
Target Species Human
Reactivity Human
Clonality Monoclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1-:1000-1:10000
Western Blot 1:1000-1:2000
Physical State Liquid (sterile filtered)
Host Isotype IgG1
Target Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Dry Ice
Purity Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c).  No reaction was observed against Human F(ab')2.
Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Human IgG F(c) fragment antibody generated in mice detects specifically human IgG. Human IgG (Fc) is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.This anti-human IgG secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 188.00
Cat# ASR2310
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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