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Anti-Human IgG F(c) Secondary Antibody
Mouse Monoclonal 3D8.H7.D4 IgG1 kappa , Unconjugated
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| Description | Anti-HUMAN IgG F(c) (MOUSE) Antibody |
|---|---|
| Host | Mouse |
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Monoclonal |
Application 
| WB, E |
| Application Note | ELISA 1-:1000-1:10000 Western Blot 1:1000-1:2000 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG1 |
| Target Isotype | IgG F(c) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Dry Ice |
|---|---|
| Purity | Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c). No reaction was observed against Human F(ab')2. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human IgG F(c) fragment antibody generated in mice detects specifically human IgG. Human IgG (Fc) is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.This anti-human IgG secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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