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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Human IgM (Fc5µ) Secondary Antibody   

Anti-Human IgM (Fc5µ) Secondary Antibody

Mouse Monoclonal 22G1.H8 IgG2b kappa , Unconjugated

  • WB - Anti-Human IgM (Fc5µ)  Secondary Antibody ASR2311
    Western Blot of Mouse anti-Human IgM Fc5 µ antibody. Lane 1: Human IgM. Lane 2: Human IgG. Lane 3: Human Fc. Lane 4: Human F(ab). Lane 5: Human F(ab)2. Lane 6: Human IgM Fc5 µ. Load: 50 ng per lane. Primary antibody: Human IgM Fc5 µ monoclonal antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicated/Observed size: 78 kDA, 78 kDa for Human IgM.
Product Information
Description Anti-HUMAN IgM (Fc5 µ) (MOUSE) Antibody
Host Mouse
Conjugate Unconjugated
Target Species Human
Reactivity Human
Clonality Monoclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:1000-1:10000
Western Blot 1:1000-1:2000
Physical State Liquid (sterile filtered)
Host Isotype IgG2b
Target Isotype IgM Fc5 µ
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Human IgM Fc5 µ was produced by repeated immunization with Human IgM Fc5 µ fragment in mice.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Dry Ice
Purity Anti-Human IgM Fc5 µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5 µ.  No reaction was observed against Human IgG, Human IgA, Human Fc, Human F(ab) or Human F(ab)2.
Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Human IgM Fc5 µ antibody generated in mice detects specifically human IgM. his anti-human IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 177.00
Cat# ASR2311
Availability: 3-5 days
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