|Description||Anti-HUMAN IgM (Fc5 µ) (MOUSE) Antibody|
|Application ||WB, E|
|Application Note||ELISA 1:1000-1:10000|
Western Blot 1:1000-1:2000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgM Fc5 µ|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Human IgM Fc5 µ was produced by repeated immunization with Human IgM Fc5 µ fragment in mice.|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Dry Ice|
|Purity||Anti-Human IgM Fc5 µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5 µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human F(ab) or Human F(ab)2.|
|Storage Condition||Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Human IgM Fc5 µ antibody generated in mice detects specifically human IgM. his anti-human IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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