|Description||Anti-HUMAN IgG F(c) (GOAT) Antibody|
|Application ||WB, E, IC|
|Application Note||ELISA 1:50,000|
Western Blot 1:10,000-1:50,000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG F(c)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Human IgG F(c) fragment|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Anti-HumanIgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab’)2.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
The anti-HumanIgG F(c) Antibody detects the Fc constant subdomain of the heavy chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin heavy chain has a constant region and variable region. The constant is used for immunomodulation, the variable region makes one half of the antigen binding F(ab).
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