F(ab')2 Anti-Cat IgG (H&L) (Peroxidase Conjugated) Secondary Antibody
Rabbit Polyclonal, Peroxidase (Horseradish)
|Description||F(ab')2 Anti-CAT IgG [H&L] (RABBIT) Antibody Peroxidase Conjugated|
|Application ||WB, E, IC|
|Application Note||ELISA 1:100,000|
Western Blot 1:5,000-1:100,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Cat IgG (H&L) was produced by repeated immunization with Cat IgG whole molecule in rabbit.|
|Reconstitution Volume||500 µL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG and Cat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically Cat IgG (H&L). This secondary antibody anti-Cat is ideal for investigators who routinely perform titration assays, westCrn-blot, immunoprecipitation and more generally immunoassays.
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