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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   HUMAN IgG Fc   


  •  - HUMAN IgG Fc ASR2419
    SDS-PAGE of Human IgG Fc. Lane 1: Non-reduced Human IgG Fc Lane 2: Non-reduced Human IgG Whole Molecule Lane 3: Non-reduced Human IgG F(ab) Fragment Lane 4: Non-reduced Human IgG F(ab')2 Fragment. Middle Lane: 5 µL OPAL Pre-stained Marker MB-210-0500. Lane 5: Reduced Human IgG Fc Lane 6: Reduced Human IgG Whole Molecule Lane 7: Reduced Human IgG F(ab) Fragment Lane 8: Reduced Human IgG F(ab')2 Fragment. Load: 1 µg per lane. Human IgG Whole Molecule, Human IgG F(ab) Fragment and Human IgG F(ab')2 Fragment ran as controls. Predicted/Observed size: Non-reduced at 50 kDa, reduced at 25 kDa/Non-reduced at 55-60 kDa, reduced at 30-33 kDa.
Product Information
Description HUMAN IgG F(c) fragment
Conjugate Unconjugated
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:5000-1:50,000
Western Blot 1:1000
Physical State Liquid (sterile filtered)
Host Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Human
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c).  No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain.
Storage Condition Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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$ 186.00
Cat# ASR2419
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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