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Rat IgM Fluorescein

  •  - Rat IgM Fluorescein  ASR2427
    SDS-PAGE of Rat IgM Whole Molecule Fluorescein Conjugated . Lane M: 3 µL Opal Prestained Marker . Lane 1: Reduced Rat IgG Whole Molecule . Lane 2: Reduced Rat IgG F(c) Fragment . Lane 3: Reduced Rat IgG F(ab) Fragment . Lane 4: Reduced Rat IgM Whole Molecule Fluorescein Conjugated . Load: 1 µg of IgG, F(c), F(ab); 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
Product Information
Description RAT IgM whole molecule Fluorescein conjugated
Conjugate Fluorescein (FITC)
FP Value 20.8 moles Fluorescein (FITC) per mole of Rat IgM
Physical State Lyophilized
Host Isotype IgM
Buffer 0.06 M Potassium Phosphate, 0.45 M Sodium Chloride, pH 7.2
Species of Origin Rat
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat Serum and anti-Rat IgM ( µ chain specific).   No reaction was observed against anti-Rat IgG F(c).  Some light chain cross reactivity will occur with anti-Rat IgG.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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$ 186.00
Cat# ASR2427
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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