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Anti-Human IgA (alpha chain) (Alkaline Phosphatase Conjugated) Secondary Antibody

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

  • DB - Anti-Human IgA (alpha chain)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR2468
    Dot Blot of Human IgA Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgA. Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng. Primary antibody: n/a Secondary antibody: Human IgA Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.
Product Information
Description Anti-HUMAN IgA (alpha chain) (GOAT) Antibody Alkaline Phosphatase Conjugated
Host Goat
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Human
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgA
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Human IgA alpha heavy chain
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against Human IgG F(c).   Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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$ 188.00
Cat# ASR2468
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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