Anti-Rabbit IgG F(ab')2 (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
|Description||Anti-RABBIT IgG F(ab')2 (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:130,000|
Western Blot 1:2,000-1:20,000
|Target Isotype||IgG F(ab')2|
|Buffer||0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG F(ab')2 fragment in goat.|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Purity||Anti-RABBIT IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Rabbit IgG F(ab')2 fragment antibody generated in goat detects specifically Rabbit IgG F(ab')2 fragment. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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