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>   home   >   Products   >   Secondary Antibodies   >   Rhodamine (TRITC)   >   F(ab')2 Anti-Rabbit IgG F(ab')2 (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody   

F(ab')2 Anti-Rabbit IgG F(ab')2 (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, Peroxidase (Horseradish)

  • WB - F(ab')2  Anti-Rabbit IgG F(ab')2  (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody ASR2528
    Western Blot of Goat anti-F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated (Pre-Adsorbed). Lane 1: Rabbit IgG F(ab')2. Lane 2: none. Load: 100 ng per lane. Secondary antibody: F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated (Pre-Adsorbed) at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rabbit IgG F(ab')2.
Product Information
Description F(ab')2 Anti-RABBIT IgG F(ab')2 (GOAT) Antibody Peroxidase Conjugated Min X Bv Hs Hu Ms Rt & Sh Serum Proteins
Host Goat
Conjugate Peroxidase (Horseradish)
Target Species Rabbit
Reactivity Rabbit
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG F(ab')2
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Rabbit IgG F(ab')2 Antibody was produced in goat by repeated immunization with Rabbit IgG F(ab')2 fragment.
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity Anti-Rabbit IgG F(ab')2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated (Pre-Adsorbed) detects F(ab')2 fragments. These fragments were generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated (Pre-Adsorbed) is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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$ 188.00
Cat# ASR2528
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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