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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   RAT IgG2a isotype control   

RAT IgG2a isotype control

Monoclonal RG2a IgG2a , Unconjugated

Product Information
Description RAT IgG2a isotype control
Conjugate Unconjugated
Clonality Monoclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note FlowCytometry 1:1000-1:5000
Physical State Liquid (sterile filtered)
Host Isotype IgG2a
Buffer 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Species of Origin Rat
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3 , IgM , and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum.  Light and heavy chain composition has been confirmed.
Storage Condition Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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RAT IgG2a isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and µ - IgM, light chain ? and ?.

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$ 186.00
Cat# ASR2532
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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