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Anti-Monkey IgA (alpha chain) (Biotin Conjugated) Secondary Antibody

Goat Polyclonal, Biotin

     
  • WB - Anti-Monkey IgA (alpha chain)  (Biotin Conjugated) Secondary Antibody ASR2543
    Western Blot of Biotin conjugated Goat anti-MONKEY IgA alpha antibody. Lane 1: Human IgA. Lane 2: none. Load: 50 ng per lane. Primary antibody: Biotin Monkey IgA alpha antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa for Monkey IgA. Other band(s): none.
    detail
  •  - Anti-Monkey IgA (alpha chain)  (Biotin Conjugated) Secondary Antibody ASR2543
    This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.
    detail
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Product Information
Description Anti-MONKEY IgA (alpha chain) (GOAT) Antibody Biotin Conjugated
Host Goat
Conjugate Biotin
Target Species Monkey
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IC, E
Application Note ELISA 1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgA
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Monkey IgA alpha heavy chain was produced by repeated immunization with Monkey IgA alpha heavy chain in goat.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum.  Specificity was confirmed by ELISA at less than 5% cross reactivity to IgM or IgG.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

Anti-Monkey IgA alpha heavy chain antibody generated in goat detects specifically Monkey IgA alpha heavy chain. This secondary antibody anti-Monkey is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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Discontinued
Cat# ASR2543
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